| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
25/04/1994 |
|
Data da última atualização: |
25/04/1994 |
|
Autoria: |
CARVALHO, L. J. C. B.; CASCARDO, J. C. M.; FERREIRA, M. A.; LOUREIRO, M. E. |
|
Afiliação: |
CENARGEN-EMBRAPA, Brasilia-DF, Brasil. |
|
Título: |
Studies on proteins and enzymes related to tuverization and starch biosynthesis in cassava roots. |
|
Ano de publicação: |
1993 |
|
Fonte/Imprenta: |
Cali, Colombia: CIAT, 1993 |
|
Páginas: |
234-238p |
|
Série: |
CIAT, Working Document,123 |
|
Idioma: |
Inglês |
|
Notas: |
International scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedinjs... Cali, Colombia: CIAT, 1993. |
|
Conteúdo: |
A method has been developed to isolate intact cassava root amyloplast through protoplast proparation. Protoplasts were obtained after incubating cassava root slices in a medium containing cellulase and pectolyase for more than 10 hours at room temperature. After purification iln a discontinuous Ficoll density fradient, the protoplasts were ruptured by forcing the suspension through modified disposable syringe. Amyloplast ilntactness was observed in a fluorescence microscopy and activity of amyloplast marker enzymes before and after rupture of the amyloplast membrane. Starch branching enzyme, ADPG-pyrophosphorylase, and starch synthetase were used as amyloplast marker enzymes. The results of the experiment are discussed in a broad starch biosynthesis and tuber ization project that is undergoinf in CENARGEN. |
|
Palavras-Chave: |
Congresso. |
|
Thesagro: |
Biossíntese; Eletroforese; Meristema. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01602naa a2200241 a 4500
001 1644383
005 1994-04-25
008 1993 bl uuuu u00u1 u #d
100 1 $aCARVALHO, L. J. C. B.
245 $aStudies on proteins and enzymes related to tuverization and starch biosynthesis in cassava roots.
260 $c1993
300 $a234-238p
490 $aCIAT, Working Document,123
500 $aInternational scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedinjs... Cali, Colombia: CIAT, 1993.
520 $aA method has been developed to isolate intact cassava root amyloplast through protoplast proparation. Protoplasts were obtained after incubating cassava root slices in a medium containing cellulase and pectolyase for more than 10 hours at room temperature. After purification iln a discontinuous Ficoll density fradient, the protoplasts were ruptured by forcing the suspension through modified disposable syringe. Amyloplast ilntactness was observed in a fluorescence microscopy and activity of amyloplast marker enzymes before and after rupture of the amyloplast membrane. Starch branching enzyme, ADPG-pyrophosphorylase, and starch synthetase were used as amyloplast marker enzymes. The results of the experiment are discussed in a broad starch biosynthesis and tuber ization project that is undergoinf in CENARGEN.
650 $aBiossíntese
650 $aEletroforese
650 $aMeristema
653 $aCongresso
700 1 $aCASCARDO, J. C. M.
700 1 $aFERREIRA, M. A.
700 1 $aLOUREIRO, M. E.
773 $tCali, Colombia: CIAT, 1993
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Hortaliças. |
|
Data corrente: |
07/12/2020 |
|
Data da última atualização: |
13/10/2021 |
|
Tipo da produção científica: |
Nota Técnica/Nota Científica |
|
Autoria: |
COSTA, C. A. da; VELOSO, J. S.; OLIVEIRA, B. F.; LOURENÇO JUNIOR, V.; REIS, A. |
|
Afiliação: |
CHRISTIANE ALMEIDA DA COSTA, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JOSIENE SILVA VELOSO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; BRUNO FERREIRA DE OLIVEIRA, UNIVERSIDADE DE BRASÍLIA; VALDIR LOURENCO JUNIOR, CNPH; AILTON REIS, CNPH. |
|
Título: |
First report of Neophloeospora maculans causing leaf spots in Morus nigra and M. alba in Brazil. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Journal of Plant Diseases and Protection, v. 128, p. 317-321, 2021. |
|
ISSN: |
1861-3829 |
|
DOI: |
https://doi.org/10.1007/s41348-020-00370-6 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Mulberry (Morus spp.), represents a set of plants grown in Brazil for production of fruits, leaves for feeding silkworms and ruminant animals, and for landscape. In 2017 mulberry leaves with symptoms of spots were collected in Santa Catarina (SC) state and in the Distrito Federal (DF). Three fungal isolates obtained from M. nigra in DF and one isolate obtained from M. alba in SC state were characterized by both molecular and morphological tools. Phylogenetic analysis showed that all isolates were grouped with isolates belonging to the species Neophloeospora maculans. All isolates were pathogenic on leaves of M. nigra seedlings under greenhouse conditions and were re-isolated in pure culture, completing the Koch?s postulates. One isolate was morphologically characterized, and it showed colonies with slow growth, grayish-white color and conidia hyaline, septated, filiform, straight or curved, measuring 56 (25?70) × 4.5 (2.5?5) that confirms the molecular identification. This work reposts M. nigra as a new host of N. maculans in Brazil, as well as expands the geographic distribution of this fungus in the country. This information is potentially relevant for establishing effective disease management strategies. |
|
Palavras-Chave: |
Morus spp; Mulberry. |
|
Thesaurus NAL: |
Phylogeny. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01930naa a2200229 a 4500
001 2127721
005 2021-10-13
008 2021 bl uuuu u00u1 u #d
022 $a1861-3829
024 7 $ahttps://doi.org/10.1007/s41348-020-00370-6$2DOI
100 1 $aCOSTA, C. A. da
245 $aFirst report of Neophloeospora maculans causing leaf spots in Morus nigra and M. alba in Brazil.$h[electronic resource]
260 $c2021
520 $aMulberry (Morus spp.), represents a set of plants grown in Brazil for production of fruits, leaves for feeding silkworms and ruminant animals, and for landscape. In 2017 mulberry leaves with symptoms of spots were collected in Santa Catarina (SC) state and in the Distrito Federal (DF). Three fungal isolates obtained from M. nigra in DF and one isolate obtained from M. alba in SC state were characterized by both molecular and morphological tools. Phylogenetic analysis showed that all isolates were grouped with isolates belonging to the species Neophloeospora maculans. All isolates were pathogenic on leaves of M. nigra seedlings under greenhouse conditions and were re-isolated in pure culture, completing the Koch?s postulates. One isolate was morphologically characterized, and it showed colonies with slow growth, grayish-white color and conidia hyaline, septated, filiform, straight or curved, measuring 56 (25?70) × 4.5 (2.5?5) that confirms the molecular identification. This work reposts M. nigra as a new host of N. maculans in Brazil, as well as expands the geographic distribution of this fungus in the country. This information is potentially relevant for establishing effective disease management strategies.
650 $aPhylogeny
653 $aMorus spp
653 $aMulberry
700 1 $aVELOSO, J. S.
700 1 $aOLIVEIRA, B. F.
700 1 $aLOURENÇO JUNIOR, V.
700 1 $aREIS, A.
773 $tJournal of Plant Diseases and Protection$gv. 128, p. 317-321, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Hortaliças (CNPH) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
